HIV RNA Planetarium Dome Movie

Tom Goddard
February 22, 2012

Movie showing molecules that bind to HIV RNA for the 2012 Biophysical Society meeting to be projected on a planetarium dome.

Flat screen projection P (512x512)
Half dome projection H (960x540) or Quicktime 1080p or MPEG4 1080p. (1920x1080, H.264).
Full dome projection D (512x512)
PNG images 2048x2048 and audio hivrna.tar (16 Gbytes, 11765 frames, 29.97 frames/second).
Separate music hiv_music.wav and narration hiv_narration.wav, text narration.txt.
Command scripts that made each segment.

January 2013. New narration by Jim Weiss, new music by Nine Inch Nails, and updated credits at end of movie.

PNG dome images 2048x2048 and audio hiv_rna_2k.tar (16 Gbytes, 11765 frames, 29.97 frames/second), and 4096x4096 hiv_rna_4k.tar (47 Gbytes).
Narration jwt2_voice.mp3 music attenuated during narration jwt2_music.mp3, mixed jwt2_mix.mp3, and unattenuated music jwt2_music_noducking.mp3.

Storyboard


Opening scene RNA wiggling. Clips P.	Fly over CD8 T-cell [1]. Clips P, D.	SIV virion with spikes between two cells. Clips P.	Core particle. Clips P.	HIV RNA dimer. Clips P, P2.	Ribosome size comparison (3u5b, 3u5d) [2]. Clips P,


Ribosome RNA decorated with 75 different proteins. (3u5c, 3u5e). Clips P,	HIV RNA decorated with ~1000 copies of nucleocapsid protein. Clips P,	Close-up of nucleocapsid binding (1a1t). Clips P.	Dimerization kissing hairpin (2d1b). Clips P.	Extended dimerization (2d1a) promoted by NC. Clips P.


tRNA primer (1fir) and reverse transcriptase (3v6d). Clips P.		DNA-RNA hybrid with RNA cleaved segments that fly away. Clips P.	Form and fly along duplex DNA. Clips P.	Circularized DNA with strand swap coloring. [5] Clips P.


Integrase binding (3os1). Clips P.	Nuclear pore (EMDB 1097), pre-integation complex must pass through. Clips P.	Chromatin with HIV integrated as 50 nucleosomes (1zbb). Clips P.	TAT binding (2w2h) at TAR RNA (2kx5) site recruits P-TEFb (3mi9) to get full length transcript. [3] Clips P.	Rev (3lph) binding to RNA (1etf) and CRM (3gjx) to export unspliced RNA from nucleus. Clips P.	Credits. Clips P.

[1] CD8 T-cells kill HIV infected but it seems are not infected themselves because they do not have the CD4 receptor. Their response against HIV is weak.
[2] Ribosome has ~5000 nucleotides, about half of a single HIV RNA strand. Has more than 80% base paired while HIV has about 40%.
[3] P-TEFb phoshorylates various sites on RNA polymerase II and other transcription complex proteins to promote transcript elongation. PubMed 22100159.
[4] NC is a nucleic acid chaperone, which means that it facilitates remodeling of nucleic acid structures to form the most thermodynamically stable conformations. PubMed 21160280.
[5] On average 3 template swaps during reverse transcription.